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1 article(s) from Johnson, Kenneth A

New standards for collecting and fitting steady state kinetic data

Kenneth A. Johnson

Beilstein J. Org. Chem. 2019, 15, 16–29, doi:10.3762/bjoc.15.2

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Graphical Abstract

Figure 1: Michaelis–Menten plot. The rate of product formation is plotted versus substrate concentration and ...

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Figure 2: Fitting data to derive k_cat and K_m. A) Synthetic data were fit to a straight line and then the obse...

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Figure 3: Fitting steady state data by simulation. A) Synthetic data from Figure 2A were fit globally to derive estima...

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Figure 4: Fitting full progress curve data by simulation. A) Synthetic data were generated and then were fit ...

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Figure 5: Optimal spacing of data points. Different scenarios for computing the distribution of data points f...

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Figure 6: Free energy profiles. Free energy profiles are shown for a three step model with different rate con...

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Figure 7: Free energy profile for DNA polymerization. Free energy profiles for a correct base pair (solid blu...

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Review

Published 02 Jan 2019

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